Isobutyrate as a precursor of n-butyrate in the biosynthesis of tylosine and fatty acids

FEMS Microbiol Lett. 1991 Nov 1;68(1):33-6. doi: 10.1016/0378-1097(91)90390-v.

Abstract

Labelled sodium isobutyrate [(CD3)2-CHCOONa] was added to the culture medium of Streptomyces fradiae and up to 14 atoms of deuterium were found to be incorporated into a molecule of tylosin aglycone (tylactone). This observation is in accordance with the data in the literature. When fatty acids were analyzed, as much as 34% of the isobutyrate incorporated into the cell was formed to be transformed into butyrate that was used for the synthesis of even, straight-chain fatty acids; 57% of the labelled isobutyrate was incorporated into the even isoacids, whereas 9% was degraded to propionate and further used for the synthesis of the odd acids.

MeSH terms

  • Butyrates / metabolism*
  • Butyric Acid
  • Fatty Acids / biosynthesis*
  • Gas Chromatography-Mass Spectrometry
  • Isobutyrates
  • Mass Spectrometry
  • Streptomyces / metabolism*
  • Tylosin / biosynthesis*

Substances

  • Butyrates
  • Fatty Acids
  • Isobutyrates
  • Butyric Acid
  • isobutyric acid
  • Tylosin