Cell adhesion, cellular tension, and cell cycle control

Methods Enzymol. 2007;426:155-75. doi: 10.1016/S0076-6879(07)26008-2.


Cooperative signaling between growth factor receptor tyrosine kinases, integrins, and the actin cytoskeleton is required for activation of the G1-phase cyclin-dependent kinases and progression through G1-phase. Increasing evidence suggests that there is cell type specificity in these cooperative interactions and that the compliance of the underlying substratum can strongly affect adhesion-dependent signaling to the cell cycle. This chapter reviews our current methods for studying how cell type specificity and changes in substratum compliance can contribute to G1-phase cell cycle control. We also describe several of our current analytical procedures.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Actins / metabolism
  • Animals
  • Cell Adhesion
  • Cell Culture Techniques
  • Cell Cycle / physiology*
  • Cell Line
  • Cell Line, Tumor
  • Cytoskeleton
  • G1 Phase / physiology
  • Humans
  • Integrins / physiology
  • Substrate Specificity


  • Actins
  • Integrins