The CD4+ T-cell response to adenovirus is focused against conserved residues within the hexon protein

J Gen Virol. 2007 Sep;88(Pt 9):2417-2425. doi: 10.1099/vir.0.82867-0.


Adenovirus is a significant pathogen in immunocompromised patients and is widely utilized as a gene delivery vector, so a detailed understanding of the human immune response to adenovirus infection is critical. This study characterized the adenovirus-specific CD4(+) T-cell response of healthy donors by incubation with whole virus or with individual hexon and fiber proteins. Adenovirus-specific CD4(+) T cells averaged 0.26 % of the CD4(+) T-cell pool and were detectable in all donors. T cells recognizing the highly conserved hexon protein accounted for 0.09 %, whereas no response was observed against the fiber protein. A panel of hexon-specific CD4(+) T-cell clones was generated and shown to lyse targets infected with adenovirus from different serotypes and species. Three CD4 T-cell epitopes are described, which map to highly conserved regions of the hexon protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / virology*
  • CD8-Positive T-Lymphocytes / immunology
  • Capsid Proteins / chemistry
  • Capsid Proteins / immunology*
  • Cell Line
  • Conserved Sequence
  • HLA Antigens / immunology
  • Humans
  • Lymphocyte Depletion
  • T-Lymphocytes / immunology*


  • Capsid Proteins
  • HLA Antigens
  • hexon capsid protein, Adenovirus