Expression of the human adenylate kinase isozymes, phosphopyruvate hydratase, 6-phosphogluconate dehydrogenase, and phosphoglucomutase-1 in man-rodent somatic cell hybrids

Biochem Genet. 1976 Feb;14(1-2):1-17. doi: 10.1007/BF00484869.

Abstract

The expression of the adenylate kinase isozymes and of phosphopyruvate hydratase was studied in man-mouse and man-hamster hybrid clones. Concordant segregation of the loci coding for AK-2 and PPH was observed in 54 of 55 primary hybrid clones, and these loci were demonstrated to be synthetic with the loci specifying PGM-1 and PGD. The pattern of expression of the four enzymes in discordant clone suggests the gene order 1pter-(PGD, PPH)-AK-2-PGM-1-centromere. In addition, AK-1 was found to be expressed independently of AK-2.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylate Kinase / metabolism*
  • Animals
  • Chromosomes / metabolism
  • Clone Cells
  • Female
  • Humans
  • Hybrid Cells / embryology*
  • Isoenzymes / metabolism*
  • Male
  • Mice
  • Phosphoglucomutase / metabolism*
  • Phosphogluconate Dehydrogenase / metabolism*
  • Phosphopyruvate Hydratase / metabolism*
  • Phosphotransferases / metabolism*
  • Translocation, Genetic

Substances

  • Isoenzymes
  • Phosphogluconate Dehydrogenase
  • Phosphotransferases
  • Adenylate Kinase
  • Phosphopyruvate Hydratase
  • Phosphoglucomutase