Quantitation of the new hypoglycaemic agent AG-EE 388 ZW in human plasma by automated high-performance liquid chromatography with electrochemical detection

J Chromatogr. 1991 Jul 17;568(1):246-52. doi: 10.1016/0378-4347(91)80360-o.


A sensitive and selective high-performance liquid chromatographic assay has been developed for a new hypoglycaemic agent AG-EE 388 ZW (I) in human plasma. Plasma samples containing I were acidified with 0.2 M hydrochloric acid, directly injected into C2 reversed-phase pre-columns, and cleaned up on-line. After pre-column switching, the substance was separated isocratically in 15 min on a C18 reversed-phase column. Quantitation was performed after amperometric detection by external standard calibration curves. The linearity of the assay was demonstrated over the therapeutic concentration range 5-200 ng/ml. between-day coefficient of variation was 9.2% at 30 ng/ml. The limit of detection was 5 ng/ml in plasma. Determination of human plasma samples after intravenous and oral administration of 1 mg of 14C-labelled I demonstrated the applicability of the assay for pharmacokinetic studies in humans.

MeSH terms

  • Administration, Oral
  • Carbamates / administration & dosage
  • Carbamates / blood*
  • Chromatography, High Pressure Liquid / methods*
  • Electrochemistry
  • Humans
  • Hypoglycemic Agents / administration & dosage
  • Hypoglycemic Agents / blood*
  • Injections, Intravenous
  • Piperidines / administration & dosage
  • Piperidines / blood*


  • Carbamates
  • Hypoglycemic Agents
  • Piperidines
  • repaglinide