Cell growth experiments using a microcalorimetric vessel equipped with oxygen and pH electrodes

J Biochem Biophys Methods. 1991 Dec;23(4):283-93. doi: 10.1016/0165-022x(91)90003-f.


A method is described for simultaneous measurements of heat production rate, oxygen activity and pH, using a 3-ml stirred microcalorimetric titration/perfusion vessel fitted with a polarographic oxygen sensor and a combination pH-electrode. Heat production rate (+/- 0.3 microW), pH (+/- 0.01) and oxygen concentration (+/- 4 mumol/l) could be measured without any cross interference between the three signals. Results from measurements on suspensions of T-lymphoma cells and E. coli are presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calorimetry* / instrumentation
  • Calorimetry* / methods
  • Cell Division*
  • Electrodes
  • Escherichia coli / growth & development
  • Humans
  • Hydrogen-Ion Concentration
  • Ligands
  • Lymphoma, T-Cell / pathology
  • Oxygen / metabolism*
  • Polarography / instrumentation
  • Tumor Cells, Cultured


  • Ligands
  • Oxygen