An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis

Nat Struct Mol Biol. 2007 Sep;14(9):875-7. doi: 10.1038/nsmb1288. Epub 2007 Aug 19.


Primases synthesize the RNA primers that are necessary for replication of the parental DNA strands. Here we report that the heterodimeric archaeal/eukaryotic primase is an iron-sulfur (Fe-S) protein. Binding of the Fe-S cluster is mediated by an evolutionarily conserved domain at the C terminus of the large subunit. We further show that the Fe-S domain is essential to the unique ability of the eukaryotic primase to start DNA replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primase / chemistry
  • DNA Primase / metabolism*
  • Electron Spin Resonance Spectroscopy
  • Iron-Sulfur Proteins / metabolism*
  • Protein Binding
  • RNA*


  • Iron-Sulfur Proteins
  • RNA primers
  • RNA
  • DNA Primase