The GluCre-ROSA26EYFP mouse: a new model for easy identification of living pancreatic alpha-cells

FEBS Lett. 2007 Sep 4;581(22):4235-40. doi: 10.1016/j.febslet.2007.07.068. Epub 2007 Aug 7.


The control of glucagon secretion by pancreatic alpha-cells is poorly understood, largely because of the difficulty to recognize living alpha-cells. We describe a new mouse model, referred to as GluCre-ROSA26EYFP (or GYY), allowing easy alpha-cell identification because of specific expression of EYFP. GYY mice displayed normal glycemic control during a fasting/refeeding test or intraperitoneal insulin injection. Glucagon secretion by isolated islets was normally inhibited by glucose and stimulated by adrenaline. [Ca(2+)](c) responses to arginine, adrenaline, diazoxide and tolbutamide, were similar in GYY and control mice. Hence, this new mouse model is a reliable and powerful tool to specifically study alpha-cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / metabolism*
  • Calcium / metabolism
  • Cell Survival
  • Glucagon / metabolism*
  • Glucagon-Secreting Cells / cytology*
  • Insulin / metabolism
  • Insulin Secretion
  • Insulin-Secreting Cells / cytology
  • Integrases / metabolism*
  • Luminescent Proteins / metabolism*
  • Mice
  • Mice, Transgenic
  • Models, Animal
  • Organ Specificity


  • Bacterial Proteins
  • Insulin
  • Luminescent Proteins
  • yellow fluorescent protein, Bacteria
  • Glucagon
  • Cre recombinase
  • Integrases
  • Calcium