Ginger ingredients reduce viability of gastric cancer cells via distinct mechanisms

Biochem Biophys Res Commun. 2007 Oct 12;362(1):218-223. doi: 10.1016/j.bbrc.2007.08.012. Epub 2007 Aug 10.

Abstract

Ginger has been used throughout the world as spice, food and traditional herb. We found that 6-gingerol, a phenolic alkanone isolated from ginger, enhanced the TRAIL-induced viability reduction of gastric cancer cells while 6-gingerol alone affected viability only slightly. 6-Gingerol facilitated TRAIL-induced apoptosis by increasing TRAIL-induced caspase-3/7 activation. 6-Gingerol was shown to down-regulate the expression of cIAP1, which suppresses caspase-3/7 activity, by inhibiting TRAIL-induced NF-kappaB activation. As 6-shogaol has a chemical structure similar to 6-gingerol, we also assessed the effect of 6-shogaol on the viability of gastric cancer cells. Unlike 6-gingerol, 6-shogaol alone reduced the viability of gastric cancer cells. 6-Shogaol was shown to damage microtubules and induce mitotic arrest. These findings indicate for the first time that in gastric cancer cells, 6-gingerol enhances TRAIL-induced viability reduction by inhibiting TRAIL-induced NF-kappaB activation while 6-shogaol alone reduces viability by damaging microtubules.

MeSH terms

  • Animals
  • Caspase 3 / metabolism
  • Caspase 7 / metabolism
  • Catechols / pharmacology*
  • Cell Line, Tumor
  • Cell Survival
  • Fatty Alcohols / pharmacology*
  • Ginger / metabolism*
  • Humans
  • Mice
  • Mice, Nude
  • Microtubules / metabolism
  • NF-kappa B / metabolism
  • Plant Extracts / pharmacology
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / pathology*
  • TNF-Related Apoptosis-Inducing Ligand / metabolism

Substances

  • Catechols
  • Fatty Alcohols
  • NF-kappa B
  • Plant Extracts
  • TNF-Related Apoptosis-Inducing Ligand
  • shogaol
  • gingerol
  • Caspase 3
  • Caspase 7