A two-step fermentation process for efficient production of penta-N-acetyl-chitopentaose in recombinant Escherichia coli

Dawei Zhang; Peng George Wang; Qingsheng Qi

doi:10.1007/s10529-007-9462-y

A two-step fermentation process for efficient production of penta-N-acetyl-chitopentaose in recombinant Escherichia coli

Biotechnol Lett. 2007 Nov;29(11):1729-33. doi: 10.1007/s10529-007-9462-y. Epub 2007 Aug 21.

Authors

Dawei Zhang¹, Peng George Wang, Qingsheng Qi

Affiliation

¹ State Key Laboratory of Microbial Technology, Life Science School, Shandong University, Jinan, 250100, P. R. China.

PMID: 17710376
DOI: 10.1007/s10529-007-9462-y

Abstract

The nodC gene from Mesorhizobium loti was cloned into E. coli, leading to production of chitin oligosaccharides (COs)-mainly penta-N-acetyl-chitopentaose. A two-step fermentation procedure was then developed which gave 930 mg CO/L with a productivity of 37 mg/l.h.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli / enzymology*
Fermentation
Glucosides / biosynthesis*
N-Acetylglucosaminyltransferases / genetics
Oligosaccharides / biosynthesis*
Recombinant Proteins / biosynthesis*
Uridine Diphosphate N-Acetylglucosamine / metabolism

Substances

Glucosides
Oligosaccharides
Recombinant Proteins
penta-N-acetyl-chitopentaose
Uridine Diphosphate N-Acetylglucosamine
N-Acetylglucosaminyltransferases