Identification of six putative human transporters with structural similarity to the drug transporter SLC22 family

Genomics. 2007 Nov;90(5):595-609. doi: 10.1016/j.ygeno.2007.03.017. Epub 2007 Aug 22.


The solute carrier family 22 (SLC22) is a large family of organic cation and anion transporters. These are transmembrane proteins expressed predominantly in kidneys and liver and mediate the uptake and excretion of environmental toxins, endogenous substances, and drugs from the body. Through a comprehensive database search we identified six human proteins not yet cloned or annotated in the reference sequence databases. Five of these belong to the SLC22 family, SLC22A20, SLC22A23, SLC22A24, SLC22A25, and SPNS3, and the sixth gene, SVOPL, is a paralog to the synaptic vesicle protein SVOP. We identified the orthologs for these genes in mouse and rat and additional homologous proteins and performed the first phylogenetic analysis on the entire SLC22 family in human, mouse, and rat. In addition, we performed a phylogenetic analysis which showed that SVOP and SV2A-C are, in a comparison with all vertebrate proteins, most similar to the SLC22 family. Finally, we performed a tissue localization study on 15 genes on a panel of 30 rat tissues using quantitative real-time polymerase chain reaction.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biological Transport
  • Brain / metabolism
  • Drug Carriers
  • Female
  • Humans
  • Male
  • Markov Chains
  • Mice
  • Molecular Sequence Data
  • Organic Anion Transporters*
  • Organic Cation Transporter 1 / genetics
  • Organic Cation Transporter 1 / metabolism
  • Phylogeny*
  • Polymerase Chain Reaction
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity


  • Drug Carriers
  • Organic Anion Transporters
  • Organic Cation Transporter 1