Periodontal therapy alters gene expression of peripheral blood monocytes

J Clin Periodontol. 2007 Sep;34(9):736-47. doi: 10.1111/j.1600-051X.2007.01113.x.


Aims: We investigated the effects of periodontal therapy on gene expression of peripheral blood monocytes.

Methods: Fifteen patients with periodontitis gave blood samples at four time points: 1 week before periodontal treatment (#1), at treatment initiation (baseline, #2), 6-week (#3) and 10-week post-baseline (#4). At baseline and 10 weeks, periodontal status was recorded and subgingival plaque samples were obtained. Periodontal therapy (periodontal surgery and extractions without adjunctive antibiotics) was completed within 6 weeks. At each time point, serum concentrations of 19 biomarkers were determined. Peripheral blood monocytes were purified, RNA was extracted, reverse-transcribed, labelled and hybridized with AffymetrixU133Plus2.0 chips. Expression profiles were analysed using linear random-effects models. Further analysis of gene ontology terms summarized the expression patterns into biologically relevant categories. Differential expression of selected genes was confirmed by real-time reverse transcriptase-polymerase chain reaction in a subset of patients.

Results: Treatment resulted in a substantial improvement in clinical periodontal status and reduction in the levels of several periodontal pathogens. Expression profiling over time revealed more than 11,000 probe sets differentially expressed at a false discovery rate of <0.05. Approximately 1/3 of the patients showed substantial changes in expression in genes relevant to innate immunity, apoptosis and cell signalling.

Conclusions: The data suggest that periodontal therapy may alter monocytic gene expression in a manner consistent with a systemic anti-inflammatory effect.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Apoptosis / genetics
  • Biomarkers / blood
  • Dental Plaque / microbiology
  • Female
  • Follow-Up Studies
  • Gene Expression Profiling*
  • Gingival Hemorrhage / blood
  • Gingival Hemorrhage / therapy
  • Gingival Recession / blood
  • Gingival Recession / therapy
  • Humans
  • Immunity, Innate / genetics
  • Male
  • Middle Aged
  • Monocytes / metabolism*
  • Periodontal Index
  • Periodontal Pocket / blood
  • Periodontal Pocket / therapy
  • Periodontitis / blood
  • Periodontitis / therapy*
  • Protein Array Analysis
  • RNA / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / genetics


  • Biomarkers
  • RNA