mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer

Mol Cancer. 2007 Aug 23;6:54. doi: 10.1186/1476-4598-6-54.

Abstract

Background: Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome.

Results: We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response.

Conclusion: This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Colorectal Neoplasms / classification*
  • Colorectal Neoplasms / genetics*
  • Female
  • Gene Expression Profiling
  • Gene Expression*
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Male
  • MicroRNAs / analysis*
  • Microsatellite Instability
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger / analysis*
  • RNA, Neoplasm / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • MicroRNAs
  • RNA, Messenger
  • RNA, Neoplasm