KLF4 regulates the expression of interleukin-10 in RAW264.7 macrophages

Biochem Biophys Res Commun. 2007 Oct 26;362(3):575-81. doi: 10.1016/j.bbrc.2007.07.157. Epub 2007 Aug 14.

Abstract

Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor. In the current study, RAW264.7 murine macrophages were treated with Escherichia coli lipopolysaccharide (LPS) to determine the expression of KLF4. A full-length cDNA or antisense oligonucleotides of KLF4 was transfected into RAW264.7 macrophages, and the expression and release of IL-10 were analyzed by RT-PCR and ELISA. The transcription and DNA binding activities of KLF4 to the IL-10 promoter were detected by the luciferase reporter and electrophoretic mobility shift assays. The results showed that treatment of RAW264.7 macrophages with LPS resulted in a dose- and time-dependent increase in KLF4 protein levels; KLF4 overexpression increased the expression of IL-10, while KLF4 inhibition decreased the expression of IL-10. The overexpression of KLF4 promoted the transcription and DNA binding activities of KLF4 to the IL-10 promoter. These results indicate that KLF4 plays an important role in regulating the expression of IL-10.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Nucleus / metabolism
  • DNA, Complementary / metabolism
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation*
  • Interleukin-10 / biosynthesis*
  • Interleukin-10 / genetics*
  • Interleukin-10 / metabolism
  • Kruppel-Like Transcription Factors / biosynthesis*
  • Luciferases / metabolism
  • Macrophages / metabolism
  • Mice
  • Oligonucleotides / chemistry
  • Promoter Regions, Genetic*
  • Transcription, Genetic
  • Transfection

Substances

  • DNA, Complementary
  • GKLF protein
  • Kruppel-Like Transcription Factors
  • Oligonucleotides
  • Interleukin-10
  • Luciferases