Genetic deficiency of complement component C8 in the rabbit: evidence of a translational defect in expression of the alpha-gamma subunit

Biochem Genet. 1991 Jun;29(5-6):271-4. doi: 10.1007/BF00590107.

Abstract

We examined the molecular basis for rabbit C8 alpha-gamma deficiency (C8D) using human C8 cDNA probes. Sequential probing of normal rabbit poly(A)+RNA revealed messages of 2.1, 1.9, and 0.8 kb for alpha, beta, and gamma, respectively. Corresponding analysis of C8D rabbit poly(A)+RNA identified messages for alpha and gamma of the same size and amounts as normal rabbits. Thus, C8 alpha-gamma deficiency in the rabbit appears to be the result of a translational rather than a transcriptional defect.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Northern
  • Complement C8 / deficiency
  • Complement C8 / genetics*
  • Gene Expression
  • Macromolecular Substances
  • Molecular Probes
  • Protein Biosynthesis*
  • RNA, Messenger / metabolism
  • Rabbits

Substances

  • Complement C8
  • Macromolecular Substances
  • Molecular Probes
  • RNA, Messenger