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, 8 (9), 632-7

Induction of Experimental Acute Ulcerative Colitis in Rats by Administration of Dextran Sulfate Sodium at Low Concentration Followed by Intracolonic Administration of 30% Ethanol

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Induction of Experimental Acute Ulcerative Colitis in Rats by Administration of Dextran Sulfate Sodium at Low Concentration Followed by Intracolonic Administration of 30% Ethanol

Yan Chen et al. J Zhejiang Univ Sci B.

Abstract

Several models of experimental ulcerative colitis have been reported previously. However, none of these models showed the optimum characteristics. Although dextran sulfate sodium-induced colitis results in inflammation resembling ulcerative colitis, an obvious obstacle is that dextran sulfate sodium is very expensive. The aim of this study was to develop an inexpensive model of colitis in rats. Sprague-Dawley rats were treated with 2% dextran sulfate sodium in drinking water for 3 d followed by an intracolonic administration of 30% ethanol. The administration of 2% dextran sulfate sodium followed by 30% ethanol induced significant weight loss, diarrhea and hematochezia in rats. Severe ulceration and inflammation of the distal part of rat colon were developed rapidly. Histological examination showed increased infiltration of polymorphonuclear leukocytes, lymphocytes and existence of cryptic abscesses and dysplasia. The model induced by dextran sulfate sodium at lower concentration followed by 30% ethanol is characterized by a clinical course, localization of the lesions and histopathological features similar to human ulcerative colitis and fulfills the criteria set out at the beginning of this study.

Figures

Fig. 1
Fig. 1
Experimental protocols of the study
Fig. 2
Fig. 2
(a) Ulcerated and hemorrhagic mucosa was found in 3 d after ethanol administration in group A while only localized hyperemia was identified in groups B and C and no damage in group D; (b) Ulceration and inflammation of the colonic mucosa of rats from group A 3 d after intracolonic administration of 30% ethanol; (c) Twenty-four hours after 30% ethanol. Ulceration can be found in group C; (d) Three days after 30% ethanol administration in group C. The damage was resolved obviously; (e) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Dysplasia and dilation of crypt (arrow) are visible; (f) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Note the crypt abscess (arrow), dysplasia and dilation of crypt
Fig. 2
Fig. 2
(a) Ulcerated and hemorrhagic mucosa was found in 3 d after ethanol administration in group A while only localized hyperemia was identified in groups B and C and no damage in group D; (b) Ulceration and inflammation of the colonic mucosa of rats from group A 3 d after intracolonic administration of 30% ethanol; (c) Twenty-four hours after 30% ethanol. Ulceration can be found in group C; (d) Three days after 30% ethanol administration in group C. The damage was resolved obviously; (e) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Dysplasia and dilation of crypt (arrow) are visible; (f) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Note the crypt abscess (arrow), dysplasia and dilation of crypt
Fig. 2
Fig. 2
(a) Ulcerated and hemorrhagic mucosa was found in 3 d after ethanol administration in group A while only localized hyperemia was identified in groups B and C and no damage in group D; (b) Ulceration and inflammation of the colonic mucosa of rats from group A 3 d after intracolonic administration of 30% ethanol; (c) Twenty-four hours after 30% ethanol. Ulceration can be found in group C; (d) Three days after 30% ethanol administration in group C. The damage was resolved obviously; (e) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Dysplasia and dilation of crypt (arrow) are visible; (f) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Note the crypt abscess (arrow), dysplasia and dilation of crypt
Fig. 2
Fig. 2
(a) Ulcerated and hemorrhagic mucosa was found in 3 d after ethanol administration in group A while only localized hyperemia was identified in groups B and C and no damage in group D; (b) Ulceration and inflammation of the colonic mucosa of rats from group A 3 d after intracolonic administration of 30% ethanol; (c) Twenty-four hours after 30% ethanol. Ulceration can be found in group C; (d) Three days after 30% ethanol administration in group C. The damage was resolved obviously; (e) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Dysplasia and dilation of crypt (arrow) are visible; (f) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Note the crypt abscess (arrow), dysplasia and dilation of crypt
Fig. 2
Fig. 2
(a) Ulcerated and hemorrhagic mucosa was found in 3 d after ethanol administration in group A while only localized hyperemia was identified in groups B and C and no damage in group D; (b) Ulceration and inflammation of the colonic mucosa of rats from group A 3 d after intracolonic administration of 30% ethanol; (c) Twenty-four hours after 30% ethanol. Ulceration can be found in group C; (d) Three days after 30% ethanol administration in group C. The damage was resolved obviously; (e) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Dysplasia and dilation of crypt (arrow) are visible; (f) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Note the crypt abscess (arrow), dysplasia and dilation of crypt
Fig. 2
Fig. 2
(a) Ulcerated and hemorrhagic mucosa was found in 3 d after ethanol administration in group A while only localized hyperemia was identified in groups B and C and no damage in group D; (b) Ulceration and inflammation of the colonic mucosa of rats from group A 3 d after intracolonic administration of 30% ethanol; (c) Twenty-four hours after 30% ethanol. Ulceration can be found in group C; (d) Three days after 30% ethanol administration in group C. The damage was resolved obviously; (e) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Dysplasia and dilation of crypt (arrow) are visible; (f) Colonic mucosa of rats in group A 7 d after intracolonic administration of 30% ethanol. Note the crypt abscess (arrow), dysplasia and dilation of crypt

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