Genetic background for SHR (SHRSR) and SHRSP and WKY lines were screened by using biochemical genetic markers and class I major histocompatibility complex (RT1) typing. There were many kinds of SHR (SHRSR), SHRSP and WKY inbred lines. The genetic variabilities within SHR (SHRSR) or SHRSP strains were small, but those within WKY strains were not so small. When SHR lines were compared with WKY lines, the allele distribution in SHR lines was different from that in WKY lines at 3-13 loci. Three genetic markers, the Es-3b, Es-4a and PT1k, were identified as specific markers of SHR (SHRSR) and SHRSP. WKY/Izm, WKY/Hos, and WKY/Jim also carried the PT1k as well as SHR lines, but WKY/N and WKY/NCrj had the RT1l haplotype.