Abstract
The high-molecular-weight rhoptry complex of Plasmodium falciparum consists of 3 non-covalently associated polypeptides of 150, 135 and 105 kDa. We present the complete nucleotide sequence of the 105-kDa (RhopH3) component of this complex derived from analysis of genomic and cDNA clones. The genomic structure is unusually complex for P. falciparum, consisting of 7 exons including 2 mini-exons of 19 and 21 amino acids. The sequence lacks tandem repeats and is conserved among several parasite isolates. B cell epitopes that induce antibody responses during natural infection were mapped to five different regions of the polypeptide.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Protozoan / genetics*
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Base Sequence
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Chromosome Mapping
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DNA, Protozoan / genetics
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Exons
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Molecular Sequence Data
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Plasmodium falciparum / genetics*
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Plasmodium falciparum / immunology*
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Protozoan Proteins / genetics
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Protozoan Proteins / immunology
Substances
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Antigens, Protozoan
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DNA, Protozoan
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Protozoan Proteins
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rhoptry associated protein, Plasmodium
Associated data
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GENBANK/M63270
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GENBANK/M63271
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GENBANK/M63272
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GENBANK/M63273
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GENBANK/M63274
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GENBANK/M63275
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GENBANK/M63276
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GENBANK/M63277
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GENBANK/M63278
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GENBANK/M65059
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GENBANK/S77766