Plant cell division is spatially organized to maintain a critical cell volume and to control growth directionality. The correct orientation of the separating cell wall is secured by means of specialized cytoskeletal structures that guide the newly formed cell plate toward a predefined cortical position. A ring of microtubules called preprophase band defines a cortical zone that corresponds to the future division plane. Coincident with the disappearance of the preprophase band microtubules, cortical actin is removed at the corresponding position, leaving an actin-depleted zone that persists throughout mitosis. Here, we review the spatial and structural organization of the cortical division zone and discuss evidence that implicate the plasma membrane in division plane establishment.