Three-dimensional chemical concentration maps in a microfluidic device using two-photon absorption fluorescence imaging

Opt Lett. 2007 Sep 1;32(17):2568-70. doi: 10.1364/ol.32.002568.


Two-photon absorption fluorescence is employed within a microfluidic device to create a three-dimensional chemical concentration map for mixing uniformity characterization. This multiphoton technique images fluorescence intensity directly and provides a simple, rapid, and readily employed route to composition characterization within microfluidic systems.