Nucleotide sequence of a human heart cDNA encoding the mitochondrial phosphate carrier

DNA Seq. 1991;2(2):133-5. doi: 10.3109/10425179109039683.


We have isolated and characterized a full length cDNA clone encoding the precursor of the human heart mitochondrial phosphate carrier protein. The entire clone is 1330 bp in length with 5'- and 3'-untranslated regions of 48 and 184 bp, respectively. The open reading frame encodes the mature protein consisting of 312 amino acids, preceded by a presequence of 49 amino acids. The amino acid sequence of the mature human phosphate carrier is 93.6, 94.2 and 33.6% identical to that of the phosphate carrier from beef, rat and yeast, respectively. Like other mitochondrial transport proteins, the human phosphate carrier has a tripartite structure. Each of the three repeats contains two hydrophobic regions which presumably span the membrane in the form of alpha-helices.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • DNA, Mitochondrial
  • Humans
  • Mitochondria, Heart / metabolism*
  • Molecular Sequence Data
  • Phosphate-Binding Proteins
  • Phosphates / metabolism*
  • Protein Precursors / genetics
  • Sequence Homology, Nucleic Acid


  • Carrier Proteins
  • DNA, Mitochondrial
  • Phosphate-Binding Proteins
  • Phosphates
  • Protein Precursors

Associated data

  • GENBANK/X60036