Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1969 Jan 3;163(3862):85-7.
doi: 10.1126/science.163.3862.85.

Haploid Plants From Pollen Grains

Haploid Plants From Pollen Grains

J P Nitsch et al. Science. .


A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-liké, structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.

Similar articles

See all similar articles

Cited by 145 articles

See all "Cited by" articles

LinkOut - more resources