Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus

FEMS Microbiol Lett. 1991 Sep 15;67(1):79-84. doi: 10.1016/0378-1097(91)90448-j.


The gene coding for the class A beta-lactamase of Citrobacter diversus has been cloned and sequenced. It contains the information for a 294-amino-acid precursor protein, including a 27-residue N-terminal signal peptide. The deduced sequence of the N-terminal portion of the mature protein is in excellent agreement with that determined by microsequencing of the protein and readily explains the pI differences observed between the naturally occurring forms I and II of the enzyme. The sequence of the mature protein exhibits a very high degree of similarity with that of the Klebsiella oxytoca class A beta-lactamase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Citrobacter / enzymology
  • Citrobacter / genetics*
  • Cloning, Molecular / methods
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction / methods
  • Sequence Homology, Nucleic Acid
  • beta-Lactamases / genetics*


  • Oligodeoxyribonucleotides
  • beta-Lactamases

Associated data

  • GENBANK/S70107
  • GENBANK/S70109
  • GENBANK/S70115
  • GENBANK/S70117
  • GENBANK/S70121
  • GENBANK/S70125
  • GENBANK/S70128
  • GENBANK/S70130
  • GENBANK/S76128
  • GENBANK/S78720