Dendritic cell expression of OX40 ligand acts as a costimulatory, not polarizing, signal for optimal Th2 priming and memory induction in vivo

J Immunol. 2007 Sep 15;179(6):3515-23. doi: 10.4049/jimmunol.179.6.3515.


Costimulatory cross-talk can occur at multiple cellular levels to potentiate expansion and polarization of Th responses. Although OX40L ligand (OX40L) is thought to play a key role in Th2 development, the critical cellular source of this molecule has yet to be identified. In this study, we demonstrate that OX40L expression by the initiating dendritic cell (DC) is a fundamental requirement for optimal induction of primary and memory Th2 responses in vivo. Analysis of the kinetics of the residual Th2 response primed by OX40L-deficient DC suggested a failure to stimulate appropriate expansion and/or survival of T cells, rather than an inability to polarize per se. The dependence upon OX40L was predominantly due to the provision of signaling through OX40 rather than retrograde signaling to the DC. Mechanistically, impaired Th2 priming in the absence of OX40L was not due to exaggerated regulation because there was no evidence of increased expansion or function of regulatory cell populations, suppression through IL-10 production, or hyporesponsiveness to secondary challenge. These data define a critical role for DC-derived OX40L in the induction and development of Th2 responses in vivo.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Cell Polarity / immunology
  • Cells, Cultured
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Dendritic Cells / transplantation
  • Gene Expression Regulation / immunology
  • Immunologic Memory*
  • Injections, Intraperitoneal
  • Ligands
  • Lymphocyte Activation / immunology*
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / deficiency
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / physiology
  • Mice
  • OX40 Ligand
  • Receptors, OX40 / physiology
  • Signal Transduction / immunology*
  • Th2 Cells / immunology*
  • Th2 Cells / metabolism*
  • Tumor Necrosis Factors / biosynthesis*
  • Tumor Necrosis Factors / deficiency
  • Tumor Necrosis Factors / genetics
  • Tumor Necrosis Factors / physiology


  • Ligands
  • Membrane Glycoproteins
  • OX40 Ligand
  • Receptors, OX40
  • Tnfrsf4 protein, mouse
  • Tnfsf4 protein, mouse
  • Tumor Necrosis Factors