Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and farnesyl diphosphate synthetase

C Chambon; V Ladeveze; M Servouse; L Blanchard; C Javelot; B Vladescu; F Karst

doi:10.1007/BF02536428

Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and farnesyl diphosphate synthetase

Lipids. 1991 Aug;26(8):633-6. doi: 10.1007/BF02536428.

Authors

C Chambon¹, V Ladeveze, M Servouse, L Blanchard, C Javelot, B Vladescu, F Karst

Affiliation

¹ Laboratoire de Biochimie et Génétique des Microorganismes Université de Poitiers, France.

PMID: 1779710
DOI: 10.1007/BF02536428

Abstract

Yeast mutant strains auxotrophic for ergosterol and blocked in mevalonate diphosphate decarboxylase (erg19) and farnesyl diphosphate (FPP) synthetase (erg20) were isolated. The main feature of the mutants blocked in FPP synthetase is their ability to excrete prenyl alcohols, such as geraniol and farnesol. The isolation of the functional ERG20 gene allowed us to show that farnesyl diphosphate synthetase could be a rate limiting enzyme in ergosterol biosynthesis in yeast.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acyclic Monoterpenes
Carboxy-Lyases / genetics
Carboxy-Lyases / metabolism*
Dimethylallyltranstransferase / genetics
Dimethylallyltranstransferase / metabolism*
Ergosterol / biosynthesis
Farnesol / metabolism
Gene Expression
Genes, Fungal
Mutation
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae / genetics
Sterols / biosynthesis*
Terpenes / metabolism

Substances

Acyclic Monoterpenes
Sterols
Terpenes
Farnesol
Dimethylallyltranstransferase
Carboxy-Lyases
pyrophosphomevalonate decarboxylase
geraniol
Ergosterol