Thiobases in Escherchia coli Transfer RNA: 2-Thiocytosine and 5-Methylaminomethyl-2-thiouracil

Science. 1968 Sep 13;161(3846):1146-7. doi: 10.1126/science.161.3846.1146.


Two new sulfur-containing pyrimidine nucleotides have been isolated from hydrolyzates of Escherichia coli transfer RNA. The structures, 2-thiocytosine and 5-methylaminomethyl-2-thiouracil, have been assigned to the bases as a result of study of ultraviolet and mass spectra. An acid-degradation product, S-methylamino-methyluracil, has been synthesized and is identical to that derived from the natural product.