Abstract
Practical methods were developed for ABO blood grouping of semen from mixed body fluids. An monoclonal antibody (P6-5H) which recognizes a tissue-specific epitope on a seminal ABO blood group substance (alpha 2-seminoglycoprotein) was used as a solid phase antibody for selective capture of the seminal ABO blood group substance. ABO blood group epitopes of secretor and non-secretor semen were detected in dilutions of 8 x 10(3)-3.2 x 10(4) and 8 x 10(3)-fold, respectively, by sandwich ELISA. ABO blood group epitopes were also detected in dilutions up to 4 x 10(3)-fold, irrespective of secretor status, by the sandwich absorption-elution test.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
ABO Blood-Group System / immunology*
-
Antibodies, Monoclonal / biosynthesis
-
Antibodies, Monoclonal / immunology*
-
Blood Grouping and Crossmatching / methods*
-
Blood Grouping and Crossmatching / standards
-
Enzyme-Linked Immunosorbent Assay / methods*
-
Enzyme-Linked Immunosorbent Assay / standards
-
Evaluation Studies as Topic
-
Forensic Medicine / methods*
-
Humans
-
Immunodominant Epitopes / immunology*
-
Prostatic Secretory Proteins*
-
Proteins / immunology*
-
Semen / immunology*
-
Seminal Plasma Proteins
Substances
-
ABO Blood-Group System
-
Antibodies, Monoclonal
-
Immunodominant Epitopes
-
Prostatic Secretory Proteins
-
Proteins
-
Seminal Plasma Proteins
-
beta-microseminoprotein