Sequencing of Avian Influenza Virus Genomes Following Random Amplification

Biotechniques. 2007 Aug;43(2):188, 190, 192. doi: 10.2144/000112529.

Abstract

Increasing surveillance for the avian influenza virus (AIV) has underscored the need for quickly and precisely characterizing isolates of this highly variable target. Random amplification, sequencing, and assembly of total RNA from nonpurified virus overcomes the need for specific primers for DNA microarray or PCR protocols.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Birds
  • Chromosome Mapping / methods*
  • Genome, Viral / genetics*
  • Influenza A virus / genetics*
  • Influenza in Birds / virology
  • Molecular Sequence Data
  • Random Amplified Polymorphic DNA Technique / methods*
  • Sequence Analysis, DNA / methods*