Ultrastructural analysis of the interaction between F-actin and respiratory syncytial virus during virus assembly

Virology. 2007 Dec 20;369(2):309-23. doi: 10.1016/j.virol.2007.08.007. Epub 2007 Sep 6.


During respiratory syncytial virus (RSV) infection there is a close physical interaction between the filamentous actin (F-actin) and the virus, involving both inclusion bodies and the virus filaments. This interaction appears to occur relatively early in the replication cycle, and can be detected from 8 h post-infection. Furthermore, during virus assembly we obtained evidence for the participation of an F-actin-associated signalling pathway involving phosphatidyl-3-kinase (PI3K). Treatment with the PI3K inhibitor LY294002 prevented the formation of virus filaments, although no effect was observed either on virus protein expression, or on trafficking of the virus glycoproteins to the cell surface. Inhibition of the activity of Rac GTPase, a down-stream effector of PI3K, by treatment with the Rac-specific inhibitor NSC23766 gave similar results. These data suggest that an intimate interaction occurs between actin and RSV, and that actin-associated signalling pathway, involving PI3K and Rac GTPase, may play an important role during virus assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / physiology*
  • Aminoquinolines / pharmacology
  • Cell Line
  • Chromones / pharmacology
  • Cytoskeleton / ultrastructure
  • Cytoskeleton / virology
  • Enzyme Inhibitors / pharmacology
  • Humans
  • Inclusion Bodies, Viral / physiology
  • Inclusion Bodies, Viral / ultrastructure
  • Microscopy, Electron, Scanning
  • Microscopy, Electron, Transmission
  • Morpholines / pharmacology
  • Phosphatidylinositol 3-Kinases / physiology
  • Phosphoinositide-3 Kinase Inhibitors
  • Pyrimidines / pharmacology
  • Respiratory Syncytial Virus, Human / physiology*
  • Respiratory Syncytial Virus, Human / ultrastructure*
  • Signal Transduction
  • Virus Assembly / physiology*
  • rac GTP-Binding Proteins / antagonists & inhibitors
  • rac GTP-Binding Proteins / physiology


  • Actins
  • Aminoquinolines
  • Chromones
  • Enzyme Inhibitors
  • Morpholines
  • NSC 23766
  • Phosphoinositide-3 Kinase Inhibitors
  • Pyrimidines
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • rac GTP-Binding Proteins