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, 858 (1-2), 129-34

Sensitive and Selective Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Aniracetam in Human Plasma

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Sensitive and Selective Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Aniracetam in Human Plasma

Jingjing Zhang et al. J Chromatogr B Analyt Technol Biomed Life Sci.

Abstract

A rapid, sensitive and selective LC-MS/MS method was developed and validated for the quantification of aniracetam in human plasma using estazolam as internal standard (IS). Following liquid-liquid extraction, the analytes were separated using a mobile phase of methanol-water (60:40, v/v) on a reverse phase C18 column and analyzed by a triple-quadrupole mass spectrometer in the selected reaction monitoring (SRM) mode using the respective [M+H]+ ions, m/z 220-->135 for aniracetam and m/z 295-->205 for the IS. The assay exhibited a linear dynamic range of 0.2-100 ng/mL for aniracetam in human plasma. The lower limit of quantification (LLOQ) was 0.2 ng/mL with a relative standard deviation of less than 15%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The validated LC-MS/MS method has been successfully applied to study the pharmacokinetics of aniracetam in healthy male Chinese volunteers.

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