Abstract
The Vibrio fischeri quorum-sensing signal N-3-oxohexanoyl-l-homoserine lactone (3OC6-HSL) activates expression of the seven-gene luminescence operon. We used microarrays to unveil 18 additional 3OC6-HSL-controlled genes, 3 of which had been identified by other means previously. We show most of these genes are regulated by the 3OC6-HSL-responsive transcriptional regulator LuxR directly. This demonstrates that V. fischeri quorum sensing regulates a substantial number of genes other than those involved in light production.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Butyrolactone / analogs & derivatives
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4-Butyrolactone / metabolism
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Aliivibrio fischeri / genetics*
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Bacterial Proteins / genetics*
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Gene Expression Profiling*
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Gene Expression Regulation, Bacterial*
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Luminescence
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Oligonucleotide Array Sequence Analysis
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Operon
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Promoter Regions, Genetic
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Protein Binding
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Quorum Sensing
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Regulon / genetics*
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Repressor Proteins / genetics*
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Trans-Activators / genetics*
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Transcription Factors / genetics*
Substances
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Bacterial Proteins
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LuxI protein, Bacteria
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Repressor Proteins
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Trans-Activators
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Transcription Factors
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LuxR autoinducer binding proteins
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N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone
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4-Butyrolactone