Abstract
The formation of the nuclear envelope (NE) around chromatin is a major membrane-remodelling event that occurs during cell division of metazoa. It is unclear whether the nuclear membrane reforms by the fusion of NE fragments or if it re-emerges from an intact tubular network of the endoplasmic reticulum (ER). Here, we show that NE formation and expansion requires a tubular ER network and occurs efficiently in the presence of the membrane fusion inhibitor GTPgammaS. Chromatin recruitment of membranes, which is initiated by tubule-end binding, followed by the formation, expansion and sealing of flat membrane sheets, is mediated by DNA-binding proteins residing in the ER. Thus, chromatin plays an active role in reshaping of the ER during NE formation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Nucleus / metabolism
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Chromatin / metabolism*
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DNA / metabolism
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Endoplasmic Reticulum / metabolism*
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Female
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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HeLa Cells
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Humans
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Male
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism
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Mice
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Microscopy, Confocal
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Models, Biological
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NIH 3T3 Cells
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Nuclear Envelope / metabolism*
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Nuclear Proteins / metabolism
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Protein Binding
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transfection
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Xenopus
Substances
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Chromatin
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Membrane Glycoproteins
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Nuclear Proteins
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POM121 protein, human
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Recombinant Fusion Proteins
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Green Fluorescent Proteins
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DNA