Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum

Nat Cell Biol. 2007 Oct;9(10):1160-6. doi: 10.1038/ncb1636. Epub 2007 Sep 9.

Abstract

The formation of the nuclear envelope (NE) around chromatin is a major membrane-remodelling event that occurs during cell division of metazoa. It is unclear whether the nuclear membrane reforms by the fusion of NE fragments or if it re-emerges from an intact tubular network of the endoplasmic reticulum (ER). Here, we show that NE formation and expansion requires a tubular ER network and occurs efficiently in the presence of the membrane fusion inhibitor GTPgammaS. Chromatin recruitment of membranes, which is initiated by tubule-end binding, followed by the formation, expansion and sealing of flat membrane sheets, is mediated by DNA-binding proteins residing in the ER. Thus, chromatin plays an active role in reshaping of the ER during NE formation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Nucleus / metabolism
  • Chromatin / metabolism*
  • DNA / metabolism
  • Endoplasmic Reticulum / metabolism*
  • Female
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • HeLa Cells
  • Humans
  • Male
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Mice
  • Microscopy, Confocal
  • Models, Biological
  • NIH 3T3 Cells
  • Nuclear Envelope / metabolism*
  • Nuclear Proteins / metabolism
  • Protein Binding
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Transfection
  • Xenopus

Substances

  • Chromatin
  • Membrane Glycoproteins
  • Nuclear Proteins
  • POM121 protein, human
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • DNA