Determination of molar ratios of vesicular stomatitis virus induced RNA species in BHK21 cells

Biochemistry. 1976 Apr 20;15(8):1663-7. doi: 10.1021/bi00653a012.

Abstract

A modified procedure for analysis of RNA in denaturing formamide-polyacrylamide slab gels containing 6 M urea is described. Using this technique, in conjucntion with fluorographic analysis, we determined molecular weights and molar ratios of the various vesicular stomatitis virus (VSV) induced RNAs in BHK21 cells. A comparison of the molar ratios of virus-specific mRHAs and their putative protein products in these cells suggests that there is little, if any, translational control of viral gene expression during acute VSV infection.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Electrophoresis, Polyacrylamide Gel
  • Genes
  • Molecular Weight
  • Protein Biosynthesis
  • RNA / biosynthesis*
  • RNA, Viral / biosynthesis*
  • Transcription, Genetic
  • Vesicular stomatitis Indiana virus / metabolism*

Substances

  • RNA, Viral
  • RNA