P450-catalyzed Steroid Hydroxylation: Assay and Product Identification by Thin-Layer Chromatography

Methods Enzymol. 1991;206:462-76. doi: 10.1016/0076-6879(91)06115-j.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Aryl Hydrocarbon Hydroxylases*
  • Autoradiography / methods
  • Carbon Radioisotopes
  • Chromatography, Thin Layer / methods
  • Cytochrome P-450 Enzyme System / analysis*
  • Cytochrome P-450 Enzyme System / metabolism
  • Hydroxylation
  • Indicators and Reagents
  • Microsomes, Liver / enzymology
  • Rats
  • Steroid Hydroxylases / analysis*
  • Steroid Hydroxylases / metabolism
  • Steroids / analysis*
  • Steroids / metabolism

Substances

  • Carbon Radioisotopes
  • Indicators and Reagents
  • Steroids
  • Cytochrome P-450 Enzyme System
  • Steroid Hydroxylases
  • Aryl Hydrocarbon Hydroxylases
  • testosterone 7-alpha-hydroxylase, hamster