Growth inhibition of cells in cultures and of vaccinia virus infected HeLa cells by derivatives of trifluorothymidine

Chemotherapy. 1976;22(3-4):221-31. doi: 10.1159/000221929.


The effects of 5-trifluoromethyluracil (F3Thy), 5-trifluoromethyl-2'-deoxyuridine (F3dThd), F3dThd-5'-P, and F3dThd-5'-methylphosphonate on the growth of HeLa cells, Novikoff hepatoma cells, L5178Y mouse leukemia cells, and on the replication of vaccinia virus in HeLa cells have been determined. F3Thy and F3dThd-5'-methylophosphonate were approximately 500-fold and 100-fold less effective, respectively, than F3dThd or F3dThd-5'-P in their inhibition of these cells. F3dThd and F3dThd-5'-P are potent inhibitors of vaccinia viral replication of HeLa cells. The nucleoside and nucleotide were 1,000-fold more inhibitory than the free base. F3dThd was nost inhibitory when added between 1 and 2 h post-infection; however, it was also somewhat inhibitory when added at later times.

MeSH terms

  • Animals
  • Carcinoma, Hepatocellular
  • Cell Division / drug effects
  • Cell Line
  • Depression, Chemical
  • HeLa Cells / drug effects*
  • Leukemia, Experimental
  • Liver Neoplasms
  • Mice
  • Thymidine / analogs & derivatives*
  • Trifluridine / administration & dosage
  • Trifluridine / analogs & derivatives
  • Trifluridine / pharmacology*
  • Vaccinia virus / drug effects*
  • Virus Replication / drug effects*


  • Trifluridine
  • Thymidine