Modulation of cell-mediated immune response in B16F-10 melanoma-induced metastatic tumor-bearing C57BL/6 mice by sulforaphane

Immunopharmacol Immunotoxicol. 2007;29(2):173-86. doi: 10.1080/08923970701511728.

Abstract

Effect of sulforaphane on cell-mediated immune response (CMI) was studied in B16F-10 melanoma-induced metastasis-bearing C57BL/6 mice. Administration of sulforaphane significantly enhanced natural killer (NK) cell activity in metastatic tumor-bearing animals (43.17% cell lysis, on day 5) and the activity was observed earlier than in tumor-bearing control animals (maximum of 9.76% cell lysis, on day 9). Antibody-dependent cellular cytotoxicity also was enhanced significantly in metastatic tumor-bearing animals (41.20% cell lysis on day 9) after sulforaphane administration compared with untreated control tumor-bearing animals (maximum of 12.62% cell lysis on day 15). An early antibody-dependent complement-mediated cytotoxicity also was observed in sulforaphane-treated tumor-bearing animals (26% cell lysis, on day 15). Administration of sulforaphane significantly enhanced the production of IL-2 and IFN-gamma in metastatic tumor-bearing animals. In addition, sulforaphane significantly downregulated the serum levels of proinflammatory cytokines such as IL-1beta, IL-6, TNF-alpha, and GM-CSF during metastasis. These data clearly suggest that sulforaphane effectively inhibited the spread of metastatic tumor cells through the stimulation of CMI, upregulation of IL-2 and IFN-gamma, and downregulation of proinflammatory cytokines IL-1beta, IL-6, TNF-alpha, and GM-CSF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody-Dependent Cell Cytotoxicity / drug effects
  • Anticarcinogenic Agents / pharmacology*
  • Complement System Proteins / physiology
  • Cytokines / metabolism
  • Granulocyte Colony-Stimulating Factor / pharmacology
  • Humans
  • Immunity, Cellular / drug effects*
  • Interferon-gamma / pharmacology
  • Interleukin-2 / pharmacology
  • Isothiocyanates
  • K562 Cells
  • Killer Cells, Natural / drug effects
  • Killer Cells, Natural / immunology
  • Male
  • Melanoma, Experimental / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Neoplasm Metastasis / immunology
  • Recombinant Proteins
  • Sulfoxides
  • Thiocyanates / pharmacology*

Substances

  • Anticarcinogenic Agents
  • Cytokines
  • Interleukin-2
  • Isothiocyanates
  • Recombinant Proteins
  • Sulfoxides
  • Thiocyanates
  • Granulocyte Colony-Stimulating Factor
  • Interferon-gamma
  • Complement System Proteins
  • sulforaphane