Chemical probes shed light on protein function

Curr Opin Struct Biol. 2007 Aug;17(4):488-94. doi: 10.1016/ Epub 2007 Sep 11.


Site-specific protein labeling with synthetic dyes is an emerging technique for live cell imaging. A protein or peptide tag fused to the protein of interest provides the means for attachment of a fluorophore or other small molecule probe, to allow non-invasive imaging of the dynamics of protein localization. The past two years have seen significant advances in such methods, the publication of a number of new tags for labeling, and the imaginative application of established techniques to tackle previously intractable biological questions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Fluorescent Dyes / chemistry
  • Humans
  • Models, Molecular
  • Molecular Probes / chemistry*
  • Protein Conformation
  • Protein Processing, Post-Translational
  • Proteins / analysis*
  • Proteins / chemistry*
  • Proteins / physiology


  • Fluorescent Dyes
  • Molecular Probes
  • Proteins