Simple and rapid colorimetric enzyme sensing assays using non-crosslinking gold nanoparticle aggregation

Chem Commun (Camb). 2007 Sep 28;(36):3729-31. doi: 10.1039/b705335e. Epub 2007 Jul 5.

Abstract

Non-crosslinking gold nanoparticle (AuNP) aggregation induced by the loss (or screen) of surface charges is applied for enzymatic activity sensing and potentially inhibitor screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / chemistry
  • Adenosine / metabolism
  • Adenosine Triphosphate / chemistry
  • Adenosine Triphosphate / metabolism
  • Alkaline Phosphatase / metabolism*
  • Animals
  • Biosensing Techniques*
  • Cattle
  • Colorimetry / methods*
  • Cross-Linking Reagents / chemistry*
  • Gold / chemistry*
  • Metal Nanoparticles / chemistry*
  • Nanotechnology / methods*

Substances

  • Cross-Linking Reagents
  • Gold
  • Adenosine Triphosphate
  • Alkaline Phosphatase
  • Adenosine