Mucin gene expression in hypertrophic adenoids

Acta Otolaryngol. 2007 Oct;127(10):1080-5. doi: 10.1080/00016480701200236.

Abstract

Conclusion: Membrane-bound mucin MUC4 represents the predominant mucin expressed in the adenoid epithelium followed by MUC5AC (gel-forming mucin). This may suggest that membrane-bound mucins could be involved in pathogen binding and immunological stimulation.

Objectives: The aim of this study was to investigate mucin expression in hypertrophic adenoids.

Materials and methods: Adenoidal samples were obtained from 12 children. The expression of eight mucin genes, MUC1-4, MUC5AC, 5B, 6 and 7 was studied by in situ hybridization utilizing digoxigenin-labelled oligonucleotide probes.

Results: The dominant mucin genes were MUC4, 3 and 5AC, while MUC1, 2, 5B and 7 were sparsely expressed and MUC6 was not expressed. Expression patterns were very different from those in the upper airways. Most samples expressed two membrane-bound mucins (MUC4 and 3) and one secretory mucin (MUC5AC).

Publication types

  • Comparative Study

MeSH terms

  • Adenoids / metabolism*
  • Adenoids / pathology*
  • Biomarkers / metabolism
  • Child
  • Child, Preschool
  • DNA / genetics*
  • Disease Progression
  • Female
  • Gene Expression*
  • Humans
  • Hypertrophy / genetics
  • Hypertrophy / metabolism
  • Hypertrophy / pathology
  • In Situ Hybridization
  • Male
  • Mucins / genetics*
  • Oligonucleotide Probes
  • Prognosis

Substances

  • Biomarkers
  • Mucins
  • Oligonucleotide Probes
  • DNA