Interface membrane fibroblasts around aseptically loosened endoprostheses express MMP-13

J Orthop Res. 2008 Feb;26(2):143-52. doi: 10.1002/jor.20494.


The objective of this article was to assess whether matrix metalloproteinase-13 (MMP-13) is produced by cells of the peri-implant interface tissues and to further characterize these cells. Tissue specimens were collected from the bone-prosthesis interface at the time of revision surgery of clinically loosened hip and knee arthroplasties (n = 27). Synovial tissues from osteoarthritic patients and young patients with mild joint deformity were used as controls (n = 6). Tissue samples were fixed in 4% PFA, decalcified with EDTA, and embedded in paraffin. Sections (4 microm) were stained with hematoxylin/eosin and for the osteoclastic marker enzyme tartrate resistant acid phosphatase. Monocytes/macrophages were characterized with a monoclonal antibody against CD68 and mRNAs encoding MMP-13 and alpha(1) collagen I (COL1A1) were detected by in situ hybridization. Cells expressing transcripts encoding MMP-13 were found in 70% of the interface tissues. These cells colocalized with a cell population expressing COL1A1 mRNA, and were fibroblastic in appearance. MMP-13 expressing cells were found in the close vicinity of osteoclasts and multinuclear giant cells. No signals for transcripts encoding MMP-13 were detected in multinuclear giant cells or in osteoclasts. Control tissues were negative for transcripts encoding MMP-13 mRNA. Fibroblasts of the interface from aseptically loosened endoprostheses selectively express MMP-13. By the expression and the release of MMP-13, these fibroblastic cells may contribute to the local degradation of the extracellular matrix and to bone resorption.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, CD / biosynthesis
  • Antigens, Differentiation, Myelomonocytic / biosynthesis
  • Bone Resorption
  • Cell Membrane / metabolism*
  • Collagen Type I / biosynthesis
  • Collagen Type I, alpha 1 Chain
  • Extracellular Matrix / metabolism
  • Female
  • Fibroblasts / metabolism*
  • Gene Expression Regulation*
  • Hip Prosthesis
  • Humans
  • Knee Prosthesis
  • Macrophages / metabolism
  • Male
  • Matrix Metalloproteinase 13 / biosynthesis*
  • Middle Aged
  • Monocytes / metabolism
  • Prosthesis Failure


  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD68 antigen, human
  • Collagen Type I
  • Collagen Type I, alpha 1 Chain
  • Matrix Metalloproteinase 13