Cross-reacting herpes simplex virus antigens in hamster and mouse cells transformed by ultraviolet light-inactivated herpes simplex virus type 2

Infect Immun. 1976 Mar;13(3):890-7. doi: 10.1128/iai.13.3.890-897.1976.


Murine and hamster cell lines, each transformed with a different strain of herpes simplex virus (HSV), were examined for cross-reacting antigens by in vitro and in vivo assays. A comparative study by the indirect immunofluorescence technique detected common cross-reacting viral antigens. Cytoplasmic fluorescence patterns were observed in the 333-8-9 hamster line, the H238 murine line, and the H238 clonal lines; these patterns were identical to the fluorescence pattern of HSV -2-infected controls when reacted with HSV antiserum. Tumor rejection studies in the BALB/c host indicated that each cell line provided immunity against a tumorigenic challenge of transformed mouse cells. The H238 clone EC1 3 provided a 53% immunity against itself at an inoculum of 10(6); the 333-8-9 line supported a 26% immunity. These data demonstrate a common HSV antigenicity between the murine and hamster transformed lines and further indicate that the HSV genome is involved in transformation.

MeSH terms

  • Animals
  • Antibodies, Viral / analysis
  • Antigens, Viral / analysis*
  • Cell Line
  • Cell Transformation, Neoplastic
  • Cricetinae
  • Cross Reactions*
  • Fluorescent Antibody Technique
  • Gammaretrovirus / immunology
  • In Vitro Techniques
  • Mice
  • Mice, Inbred BALB C
  • Simplexvirus / immunology*
  • Simplexvirus / radiation effects
  • Ultraviolet Rays


  • Antibodies, Viral
  • Antigens, Viral