Yeast surface display for protein engineering and characterization

Curr Opin Struct Biol. 2007 Aug;17(4):467-73. doi: 10.1016/ Epub 2007 Sep 17.


Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein-protein interactions, represents a significant recent advance.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism
  • Humans
  • Peptide Library
  • Protein Conformation
  • Protein Engineering / methods*
  • Protein Interaction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Solubility


  • Enzymes, Immobilized
  • Peptide Library