Abstract
Cycloheximide, ketoconazole, or preexposure of organisms to cytochalasin D prevented Balamuthia mandrillaris-associated cytopathogenicity in human brain microvascular endothelial cells, which constitute the blood-brain barrier. In an assay for inhibition of cyst production, these three agents prevented the production of cysts, suggesting that the biosynthesis of proteins and ergosterol and the polymerization of actin are important in cytopathogenicity and encystment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism
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Amebicides / pharmacology*
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Amoeba / drug effects*
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Amoeba / growth & development
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Amoeba / metabolism
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Animals
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Cell Line
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Cell Survival / drug effects
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Cycloheximide / pharmacology
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Cytochalasin D / pharmacology
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Cytoskeleton / metabolism
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Dose-Response Relationship, Drug
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Endothelial Cells / cytology
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Endothelial Cells / drug effects*
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Endothelial Cells / parasitology
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Ergosterol / biosynthesis
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Humans
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Ketoconazole / pharmacology
Substances
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Actins
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Amebicides
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Cytochalasin D
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Cycloheximide
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Ketoconazole
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Ergosterol