Fmoc-based solid phase chemical synthesis of 71-meric neuregulin 1-beta1, an epidermal growth factor-like domain

J Pept Sci. 2008 Mar;14(3):261-6. doi: 10.1002/psc.916.

Abstract

The human neuregulin 1-beta1 (NRG1-beta1, amino acid residues 176-246) was chemically synthesized by Fmoc-based solid phase peptide synthesis (SPPS) followed by folding in a redox buffer. The biological activity of the synthesized NRG1-beta1 was confirmed by ligand-induced tyrosine phosphorylation on Chinese hamster ovary (CHO) cells expressing ErbB-4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry*
  • Epidermal Growth Factor / chemistry*
  • Epidermal Growth Factor / physiology
  • Fluorenes / chemistry*
  • Humans
  • Nerve Tissue Proteins / chemistry*
  • Neuregulin-1
  • Peptides / chemical synthesis*
  • Polymers / chemical synthesis*
  • Polymers / chemistry
  • Protein Structure, Tertiary

Substances

  • Amino Acids
  • Fluorenes
  • N(alpha)-fluorenylmethyloxycarbonylamino acids
  • NRG1 protein, human
  • Nerve Tissue Proteins
  • Neuregulin-1
  • Peptides
  • Polymers
  • Epidermal Growth Factor