Fmoc-based solid phase chemical synthesis of 71-meric neuregulin 1-beta1, an epidermal growth factor-like domain

Taeko Kakizawa; Shizuyo Koide-Yoshida; Tooru Kimura; Hiromasa Uchimura; Yoshio Hayashi; Kazuki Saito; Yoshiaki Kiso

doi:10.1002/psc.916

Fmoc-based solid phase chemical synthesis of 71-meric neuregulin 1-beta1, an epidermal growth factor-like domain

J Pept Sci. 2008 Mar;14(3):261-6. doi: 10.1002/psc.916.

Authors

Taeko Kakizawa¹, Shizuyo Koide-Yoshida, Tooru Kimura, Hiromasa Uchimura, Yoshio Hayashi, Kazuki Saito, Yoshiaki Kiso

Affiliation

¹ Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century COE Program, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8412, Japan.

PMID: 17880034
DOI: 10.1002/psc.916

Abstract

The human neuregulin 1-beta1 (NRG1-beta1, amino acid residues 176-246) was chemically synthesized by Fmoc-based solid phase peptide synthesis (SPPS) followed by folding in a redox buffer. The biological activity of the synthesized NRG1-beta1 was confirmed by ligand-induced tyrosine phosphorylation on Chinese hamster ovary (CHO) cells expressing ErbB-4.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / chemistry*
Epidermal Growth Factor / chemistry*
Epidermal Growth Factor / physiology
Fluorenes / chemistry*
Humans
Nerve Tissue Proteins / chemistry*
Neuregulin-1
Peptides / chemical synthesis*
Polymers / chemical synthesis*
Polymers / chemistry
Protein Structure, Tertiary

Substances

Amino Acids
Fluorenes
N(alpha)-fluorenylmethyloxycarbonylamino acids
NRG1 protein, human
Nerve Tissue Proteins
Neuregulin-1
Peptides
Polymers
Epidermal Growth Factor