Novel mating type-dependent transcripts at the mating type locus in Magnaporthe oryzae

Gene. 2007 Nov 15;403(1-2):6-17. doi: 10.1016/j.gene.2007.06.015. Epub 2007 Jul 3.


The mating type locus (MAT1) of Magnaporthe oryzae has similar structural organization to MAT in other ascomycetes and encodes the mating type genes MAT1-1-1 with an alpha-box motif and MAT1-2-1 with an HMG-box motif in the MAT1-1 and MAT1-2 idiomorphs, respectively. Sequence and expression analyses of the MAT1 locus indicated a second open reading frame (ORF), MAT1-1-2, in the MAT1-1 idiomorph, and novel mating-type dependent ORFs (MAT1-1-3 and MAT1-2-2) at the locus. The MAT1-1-3 ORF initiated within the MAT1-1 idiomorph while the MAT1-2-2 ORF initiated at the border of the MAT1-2 idiomorph with both ORFs sharing most of their reading frames in the MAT1 flanking region. This suggests that the encoded proteins (MAT1-1-3 and MAT1-2-2) should be similar in their primary structures but can be distinguished by distinct N-termini with amino acids of 1 and 32, respectively, in each mating type. A CT dinucleotide repeat, (CT)n, present in the upstream region of MAT1-1-3, was polymorphic among the isolates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions
  • Amino Acid Sequence
  • Base Pairing
  • Base Sequence
  • Cloning, Molecular
  • Crosses, Genetic
  • DNA Primers
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Dinucleotide Repeats
  • Fungal Proteins / genetics
  • Genes, Fungal*
  • Genes, Mating Type, Fungal*
  • HMG-Box Domains
  • Magnaporthe / genetics*
  • Magnaporthe / growth & development
  • Molecular Sequence Data
  • Nucleic Acid Amplification Techniques
  • Open Reading Frames
  • Polymerase Chain Reaction / methods
  • Polymorphism, Genetic
  • RNA, Messenger / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription Initiation Site
  • Transcription, Genetic


  • 5' Untranslated Regions
  • DNA Primers
  • DNA, Fungal
  • Fungal Proteins
  • RNA, Messenger