Effects of scutellarin on PKCgamma in PC12 cell injury induced by oxygen and glucose deprivation

Acta Pharmacol Sin. 2007 Oct;28(10):1573-9. doi: 10.1111/j.1745-7254.2007.00502.x.


Aim: To evaluate the neuroprotective effect and mechanisms of scutellarin (Scu) against PC12 cell injury after oxygen and glucose deprivation followed by reperfusion (OGD-Rep).

Methods: Undifferentiated rat pheochromocytoma PC12 cells, exposed to oxygen and glucose deprivation followed by reperfusion (OGD-Rep), used as an in vitro model of ischemia/reperfusion. Cell survival was evaluated by diphenyltetrazolium bromide (MTT) assay and the amount of LDH release was determined using assay kits. [Ca2+](i) was monitored using a fluorescent Ca2+-sensitive dye Fura-2 acetoxymethyl ester. Cell apoptosis was detected by a DNA ladder and by flow cytometric detection. The expression of protein kinase C (PKC)gamma was determined using both RT-PCR and Western blotting. The translocation of PKCgamma was assayed by subcellular fractionation and Western blotting.

Results: OGD-Rep injury significantly elevated the level of LDH release, [Ca2+](i), mRNA expression and the translocation of PKCgamma compared in the PC12 cells with those of the normal group. Scu (10-100 micromol/L) exerted a protective effect against OGD-Rep injury by reducing LDH release, [Ca2+](i), the percent of apoptosis, and the translocation of PKCgamma.

Conclusion: Scu inhibits the increase of [Ca2+](i) and the activation of PKCgamma, exerting protective effects against PC12 cell injury induced by OGD-Rep.

MeSH terms

  • Animals
  • Apigenin / isolation & purification
  • Apigenin / pharmacology*
  • Apoptosis / drug effects*
  • Biological Transport / drug effects
  • Blotting, Western
  • Calcium / metabolism
  • Cell Hypoxia
  • Cell Membrane / metabolism
  • Cell Nucleus / metabolism
  • Cytosol / drug effects
  • Cytosol / metabolism
  • Erigeron / chemistry
  • Flow Cytometry
  • Glucose / metabolism
  • Glucose / pharmacology
  • Glucuronates / isolation & purification
  • Glucuronates / pharmacology*
  • L-Lactate Dehydrogenase / metabolism
  • Neuroprotective Agents / isolation & purification
  • Neuroprotective Agents / pharmacology*
  • Oxygen / metabolism
  • Oxygen / pharmacology
  • PC12 Cells
  • Plants, Medicinal / chemistry
  • Protein Kinase C / biosynthesis*
  • Protein Kinase C / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction


  • Glucuronates
  • Neuroprotective Agents
  • RNA, Messenger
  • scutellarin
  • Apigenin
  • L-Lactate Dehydrogenase
  • protein kinase C gamma
  • Protein Kinase C
  • Glucose
  • Oxygen
  • Calcium