Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside

Biochem Biophys Res Commun. 2007 Nov 23;363(3):472-8. doi: 10.1016/j.bbrc.2007.08.155. Epub 2007 Sep 4.

Abstract

Our aim was to obtain a viable and easily available dermal substitute (DS) for the definitive coverage of full-thickness burns. A DS composed of a collagen-glycosaminoglycan-chitosan dermal matrix (DM) colonized with foreskin fibroblasts (FF) is described. FF-colonized DS were compared to the DM seeded with adult dermal fibroblasts (DF). FF-colonized DS expressed more fibrillin and tropoelastin than that with DF. Reconstructed skin obtained with both FF- and DF-colonized DS similarly expressed laminin-5 and collagen VII at the dermal-epidermal junction. Both FF- and DF-colonized DS produced cutaneous wound healing mediators in a dose-dependent manner in the presence of platelet lysate. After freeze-thawing, the FF-colonized DS were recovered in culture and retained their ability to produce vascular endothelial growth factor. Grafting of DS into nude rats achieved a complete healing of a dermal-epidermal lesion with a good epidermalization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Burns / physiopathology
  • Burns / surgery
  • Burns / therapy*
  • Cell Survival
  • Chemokines / metabolism
  • Dermis / growth & development
  • Dermis / metabolism
  • Dermis / surgery
  • Extracellular Matrix Proteins / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Freezing
  • Humans
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Male
  • Microscopy, Fluorescence
  • Rats
  • Rats, Nude
  • Skin / cytology
  • Skin / metabolism
  • Skin Transplantation / methods
  • Skin, Artificial*
  • Time Factors
  • Tissue Engineering / methods*
  • Transplantation, Heterologous
  • Wound Healing

Substances

  • Chemokines
  • Extracellular Matrix Proteins
  • Intercellular Signaling Peptides and Proteins