Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: a note of warning

FEMS Yeast Res. 2008 Feb;8(1):2-5. doi: 10.1111/j.1567-1364.2007.00315.x. Epub 2007 Sep 24.

Abstract

The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trp1 strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldose-Ketose Isomerases / genetics*
  • Aldose-Ketose Isomerases / physiology
  • Biomarkers*
  • Phenotype
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / physiology

Substances

  • Biomarkers
  • Saccharomyces cerevisiae Proteins
  • Aldose-Ketose Isomerases
  • TRP1 protein, S cerevisiae