HDAC10 promoter polymorphism associated with development of HCC among chronic HBV patients

Biochem Biophys Res Commun. 2007 Nov 23;363(3):776-81. doi: 10.1016/j.bbrc.2007.09.026. Epub 2007 Sep 18.


Histone deacetylases (HDACs) are key enzymes responsible for the removal of acetyl groups from acetylated histone and non-histone proteins, and play important roles in various biological processes including transcription regulation and DNA repair. In this study, we identified 22 sequence variants by direct DNA sequencing in 24 individuals and five common variant were selected for genotyping in larger-scale subjects (n=1095). Statistical analysis revealed that HDAC10-589C>T was significantly associated with HCC occurrence among chronic HBV patients (OR=2.39, P(cor)=0.04) as well as HCC acceleration among chronic HBV patients (RH=1.97, Pcor=0.002). Functional assay also revealed that luciferase activity of "T" allele was significantly higher than that of "C" allele of HDAC10-589C>T (P=0.023). These results suggest that the "T" allele of HDAC10-589C>T affect on the increased transcription activity, and might accelerate HCC development through increased expression of HDAC10.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Hepatocellular / etiology
  • Carcinoma, Hepatocellular / genetics*
  • Cell Line, Tumor
  • DNA Mutational Analysis / statistics & numerical data
  • Female
  • Gene Frequency
  • Genotype
  • Haplotypes
  • Hepatitis B virus
  • Hepatitis B, Chronic / complications*
  • Hepatitis B, Chronic / virology
  • Histone Deacetylases / genetics*
  • Humans
  • Kaplan-Meier Estimate
  • Liver Neoplasms / etiology
  • Liver Neoplasms / genetics*
  • Luciferases / genetics
  • Luciferases / metabolism
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Promoter Regions, Genetic / genetics*
  • Proportional Hazards Models
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Transfection


  • Recombinant Fusion Proteins
  • Luciferases
  • HDAC10 protein, human
  • Histone Deacetylases