Germ-line Transformation of the Mexican Fruit Fly

Insect Mol Biol. 2007 Oct;16(5):573-80. doi: 10.1111/j.1365-2583.2007.00752.x.

Abstract

Germ-line transformation of a major agricultural pest, the Mexican fruit fly (Anastrepha ludens Loew, Mexfly), was achieved using composite piggyBac transposable elements marked with green, yellow and red fluorescent proteins (CopGreen, PhiYFP and J-Red). We also investigated the possibility of generating transposon-free insertions, in order to address potential concerns relating to proposed field use of transgenic Mexfly. We describe a highly efficient method for transforming Mexfly, compare efficiency of piggyBac terminal sequences for transformation and also describe the derivation of a transposon-free insertion line. The development of an efficient transformation system for Mexfly holds great promise for improved applications of the sterile insect technique, a major component of the present control measures for this economically important pest species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Transposable Elements*
  • Embryo, Nonmammalian
  • Female
  • Genetic Engineering / methods*
  • Genetic Markers
  • Germ Cells*
  • Microinjections
  • Plasmids
  • Tephritidae / embryology
  • Tephritidae / genetics*
  • Transformation, Genetic*

Substances

  • DNA Transposable Elements
  • Genetic Markers